Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OPA1 in COS cells (Prof. Mustapha Oulad-Abdelghani, IGBMC, France).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated OPA1 from mouse brain tissue lysate (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).

This Anti-OPA1 antibody, clone 1OPA-1A8 is validated for use in western blotting, ICC & IP for the detection of OPA1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

OPA1 (optic atrophy 1) is a dynamin-related GTPase that plays a role in genome preservation and mitochondrial morphology. Localization of OPA1 is thought to be anchored to the inner membrane of the mitochondria. OPA1 is highly expressed in inner and outer retinal neurons and is important for normal mitochondrial function in those cells. Mutations in OPA1 are the major cause of autosomal dominant optic atrophy. Recent research has found that OPA1 also has the ability to protect neurons from excitotoxic injury and could be a potential clinical target to increase endurance of neurons post brain damage.

Immunogen

Recombinant protein corresponding to mouse OPA1.

Physical form

Mouse monoclonal IgG1κ ascites containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OPA1 in 10 µg of human brain tissue lysate.

Specificity

This antibody detects six known isoforms of OPA1 (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~90 and ~100 kDa observed. Six isoforms between 80 kDa and 100 kDa are known to exist (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).